For the second game running Coach Jose Peseiro has decided to stick with the 3-4-3 formation for the Super Eagles starting 11 against Guinea Bissau.
Kenneth Omeruo captains the team on his 62nd international cap for the country after Troost Ekong was ruled out.
Calvin Bassey and Ajayi are the other central defenders making up the three at the back for the tie scheduled for the Stade Felix Houphouet Boigny.
Meanwhile, Joe Aribo and Bright Osayi also get their first AFCON start after substitute appearances in the first two games.
Eagles starting 11 also features Ola Aina and Stanley Nwabali, starting for the third consecutive game.
Nigeria is currently second on the group table with 4 points from two games.
A win against host Cote d’Ivoire and a surprising draw against Equatorial Guinea in the first game.
The Super Eagles need at least a draw to qualify for the next round of the 2023 AFCON in Cote d’Ivoire.
Guinea Bissau Vs Super Eagles Starting 11:
Stanley Bobo Nwabali
Ola Aina
Bright Osayi Samuel
Kenneth Omeruo (C)
Semi Ajayi
Calvin Bassey
Frank Onyeka
Joe Aribo
Moses Simon
Samuel Chukwueze
Victor Osimhen
Elsewhere, in Group A Cote d’Ivoire and Equatorial Guinea have a date with destiny. Both teams have huge hurdles to cross. The Elephants have to save face as host, while the Thunders know they have a great chance to advance to the next round if they contain their opponents tonight.